5DS2
Core domain of the class I small heat-shock protein HSP 18.1 from Pisum sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.843 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.060, 89.060, 142.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.130 - 1.850 |
| R-factor | 0.2054 |
| Rwork | 0.203 |
| R-free | 0.24487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.746 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.130 |
| High resolution limit [Å] | 1.850 |
| Rmeas | 0.041 |
| Number of reflections | 56563 |
| <I/σ(I)> | 35.12 |
| Completeness [%] | 99.9 |
| Redundancy | 10.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | c18.1 crystallised in 200 mM Li2SO4, 100 mM Tris-HCl, pH8.5, 30 % (w/v) PEG 400 in hanging drop plates at room temperature. |
