5DRV
Crystal structure of the G3BP2 NTF2-like domain in complex with a peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.87 |
Spacegroup name | H 3 2 |
Unit cell lengths | 94.850, 94.850, 114.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.641 - 2.750 |
R-factor | 0.2503 |
Rwork | 0.245 |
R-free | 0.29910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fcm |
RMSD bond length | 0.002 |
RMSD bond angle | 0.577 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.146 | 2.120 |
Number of reflections | 5273 | |
<I/σ(I)> | 21.9 | 2 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 19 | 20.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.9 M tri-sodium citrate, 20 % glycerol, 0.1 M sodium cacodylate (pH 6.4) |