5DRV
Crystal structure of the G3BP2 NTF2-like domain in complex with a peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.87 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.850, 94.850, 114.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.641 - 2.750 |
| R-factor | 0.2503 |
| Rwork | 0.245 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fcm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.577 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.146 | 2.120 |
| Number of reflections | 5273 | |
| <I/σ(I)> | 21.9 | 2 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 19 | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.9 M tri-sodium citrate, 20 % glycerol, 0.1 M sodium cacodylate (pH 6.4) |
