5DRK

2.3 Angstrom Structure of CPII, a nitrogen regulatory PII-like protein from Thiomonas intermedia K12, bound to ADP, AMP and bicarbonate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-13
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.510, 75.610, 79.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.910 - 2.380
R-factor	0.2439
Rwork	0.244
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.723
Data scaling software	XSCALE
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.910		2.440
High resolution limit [Å]	2.380	10.630	2.380
Rmerge	0.113	0.028	0.946
Rmeas	0.118	0.029	0.990
Total number of observations	160664
Number of reflections	13864	187	910
<I/σ(I)>	20.51	69.46	3.06
Completeness [%]	99.7	98.9	96.1
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.2	298	0.2 M Sodium dihydrogen Phosphate monohydrate, 20% w/v PEG 3350