5DRH
Crystal structure of apo C-As lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 44.120, 44.120, 117.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.307 - 2.270 |
| R-factor | 0.2311 |
| Rwork | 0.230 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c4p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.829 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.079 | 0.413 |
| Number of reflections | 5898 | |
| <I/σ(I)> | 24.1 | 8.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M sodium acetate, 0.1 M Tris-HCl, pH 8.5, 30% PEG4000 |
