5DQU

Crystal Structure of Cas-DNA-10 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	71.095, 195.775, 194.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.500
R-factor	0.2657
Rwork	0.265
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p6i
RMSD bond length	0.010
RMSD bond angle	1.678
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.660
High resolution limit [Å]	4.500	9.670	4.500
Rmerge	0.154	0.123	0.688
Rmeas	0.169	0.135	0.752
Rpim	0.067	0.054	0.300
Total number of observations	106617
Number of reflections	17232
<I/σ(I)>	6.4
Completeness [%]	100.0	99.8	100
Redundancy	6.2	6	6.1
CC(1/2)		0.959	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	8% PEG8000,100mM Tris-HCl