5DQR
The crystal structure of Arabidopsis 7-hydroxymethyl chlorophyll a reductase (HCAR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 |
Unit cell lengths | 89.105, 89.105, 273.312 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.610 - 2.700 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24600 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.079 | 0.512 |
Number of reflections | 66528 | |
<I/σ(I)> | 11.6 | 2.3 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M MgCl2, 0.1M Tris-HCl pH 8.5, 25%PEG3350(w/v), 3% EG(v/v) |