5DPS

Crystal structure of PLEKHM1 LIR-fused human GABARAP_2-117

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 31
Unit cell lengths	73.806, 73.806, 74.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.903 - 2.000
R-factor	0.1859
Rwork	0.184
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.903	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	30464
<I/σ(I)>	10.3
Completeness [%]	99.2	98.6
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	278	4% v/v Tacsimate pH 8.0, 12% w/v Polyethylene glycol 3,350