5DPQ

Crystal Structure of E72A mutant of domain swapped dimer Human Cellular Retinol Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2015-06-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	62.238, 72.901, 60.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.453 - 1.775
R-factor	0.1778
Rwork	0.174
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.012
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.050
Number of reflections	27242
<I/σ(I)>	75.13	2.76
Completeness [%]	100.0	100
Redundancy	1.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	30% PEG 4000, 0.1 M sodium acetate, 0.1 M ammonium acetate, pH 4.6, EVAPORATION, temperature 298K