5DPH
sfGFP mutant - 149 p-cyano-L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 47.270, 47.270, 344.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.000 - 1.420 |
| R-factor | 0.1832 |
| Rwork | 0.180 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 1.450 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.042 | 1.270 |
| Number of reflections | 81014 | |
| <I/σ(I)> | 45.1 | 2.22 |
| Completeness [%] | 99.4 | 94.7 |
| Redundancy | 36.9 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 20% PEG 4000, 0.21 M MgCl2-6H2O |
