5DPG
sfGFP mutant - 133 p-cyano-L-phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.774, 68.057, 53.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.331 - 1.850 |
R-factor | 0.1509 |
Rwork | 0.149 |
R-free | 0.19200 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.353 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.065 | 0.504 |
Number of reflections | 19485 | |
<I/σ(I)> | 25.9 | 2.9 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 22% PEG 3350, 2% Tacsimate pH 6, 0.1 M Bis-Tris pH 6.5 |