5DOX

Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution

This is a non-PDB format compatible entry.

Replaces:  4Z3R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	209.510, 448.290, 619.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	309.685 - 3.100
R-factor	0.2347
Rwork	0.232
R-free	0.28390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	309.685	309.685	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.227	0.025	2.118
Rmeas	0.252	0.028	2.346
Total number of observations	5754441
Number of reflections	1041680	12175	76735
<I/σ(I)>	7.75	42.87	1.01
Completeness [%]	99.9	98	99.9
Redundancy	5.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME