5DOX
Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution
This is a non-PDB format compatible entry.
Replaces: 4Z3RExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.510, 448.290, 619.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 309.685 - 3.100 |
R-factor | 0.2347 |
Rwork | 0.232 |
R-free | 0.28390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4o |
RMSD bond length | 0.003 |
RMSD bond angle | 0.669 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 309.685 | 309.685 | 3.180 |
High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
Rmerge | 0.227 | 0.025 | 2.118 |
Rmeas | 0.252 | 0.028 | 2.346 |
Total number of observations | 5754441 | ||
Number of reflections | 1041680 | 12175 | 76735 |
<I/σ(I)> | 7.75 | 42.87 | 1.01 |
Completeness [%] | 99.9 | 98 | 99.9 |
Redundancy | 5.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |