5DOI

Crystal structure of Tetrahymena p45N and p19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.1272
Spacegroup name	P 1 21 1
Unit cell lengths	44.192, 125.828, 180.167
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	44.192 - 2.200
R-factor	0.2323
Rwork	0.226
R-free	0.26440
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.162
Data scaling software	Aimless (0.3.8)
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.830	125.830	2.460
High resolution limit [Å]	2.200	4.920	2.200
Rmerge	0.104	0.051	0.544
Rpim	0.050	0.025	0.263
Total number of observations	512980	45351	145833
Number of reflections	99847
<I/σ(I)>	10.8	27.3	2.9
Completeness [%]	99.9	99.6	99.9
Redundancy	5.1	5	5.1
CC(1/2)	0.998	0.998	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	4.1 M LiCl, 50 mM HEPES sodium pH7.0, 10 mM MgCl2