5DOF
Crystal structure of Tetrahymena p19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2014-07-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.732, 128.631, 75.328 |
Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
Resolution | 39.370 - 1.700 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.22010 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.117 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
Rmerge | 0.075 | 0.043 | 0.657 |
Rmeas | 0.081 | 0.046 | 0.712 |
Rpim | 0.029 | 0.017 | 0.270 |
Total number of observations | 619166 | ||
Number of reflections | 83371 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.1 | 100 | 82.8 |
Redundancy | 7.4 | 7.3 | 6.7 |
CC(1/2) | 0.998 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 277 | 4.0 M sodium formate pH 7.0, 2% (v/v) PEG 400 |