5DOC
Crystal structure of the Human Cytomegalovirus UL53 subunit of the NEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.800, 50.300, 63.000 |
| Unit cell angles | 91.00, 103.00, 108.00 |
Refinement procedure
| Resolution | 61.060 - 1.940 |
| R-factor | 0.18545 |
| Rwork | 0.182 |
| R-free | 0.21983 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.437 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.060 | |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 26803 | |
| <I/σ(I)> | 27.3 | |
| Completeness [%] | 94.0 | 72.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M Hepes, 10-15 % PEG 3350, and 0.15 M ammonium acetate |
