5DO8
1.8 Angstrom crystal structure of Listeria monocytogenes Lmo0184 alpha-1,6-glucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.123, 103.937, 193.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.15525 |
Rwork | 0.154 |
R-free | 0.18837 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uok |
RMSD bond length | 0.009 |
RMSD bond angle | 1.380 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.109 | 0.741 |
Number of reflections | 162894 | |
<I/σ(I)> | 17.2 | 2.6 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 300 | Crystallization condition: JCSG+ H8 (Qiagen) 0.1 M Am Acetate, 0.1 M Bis-tris (pH 5.5), 17% Peg 10000 Protein condition: 9 mg/ml + 10 mM Tris (pH 8.3), 500 mM NaCl, 50 mM glucose |