5DNY

Structure of the ATPrS-Mre11/Rad50-DNA complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
格子定数 [Å]	84.303, 130.111, 166.656
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	40.865 - 3.110
R因子	0.2239
Rwork	0.221
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.567
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.10-2152_1745))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	3.150
分解能 [Å] (高)	3.110	3.100
独立反射数	32303
<I/σ(I)>	18.7
完全性 [%]	96.4	97
冗長性	6.3	6.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 400, Tris-HCl, Trimethylamine hydrochloride