5DNV
Crystal structure of KAI2-like protein from Striga (apo state 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.000, 76.000, 180.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.880 - 2.650 |
| R-factor | 0.21017 |
| Rwork | 0.207 |
| R-free | 0.26557 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.093 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.880 | |
| High resolution limit [Å] | 2.590 | |
| Rmerge | 0.630 | |
| Number of reflections | 9559 | |
| <I/σ(I)> | 25 | 5.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 20.2 | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | Sodium formate, Tris |
