5DNU
Crystal structure of Striga KAI2-like protein in complex with karrikin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-06-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 75.757, 75.757, 181.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 1.200 |
| R-factor | 0.14089 |
| Rwork | 0.140 |
| R-free | 0.15539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dnw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.390 | |
| Number of reflections | 96642 | |
| <I/σ(I)> | 97.8 | 5.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 20.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | Sodium formate, Tris |
