5DNS
t1428 loop variant in P3221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.570, 104.570, 87.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.560 - 3.561 |
| R-factor | 0.1883 |
| Rwork | 0.184 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.545 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.500 | 3.650 |
| High resolution limit [Å] | 3.560 | 3.560 |
| Rmerge | 0.100 | 0.900 |
| <I/σ(I)> | 20.6 | 3.59 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290 | 0.2 M sodium bromide, 20% w/v PEG 3350 |
