5DNO
Crystal structure of Mmi1 YTH domain complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 77.527, 77.527, 65.529 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.760 - 1.800 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.760 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.149 | 0.602 |
| Number of reflections | 20848 | |
| <I/σ(I)> | 25.4 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 11.5 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 18% (w/v) PEG 2000 |
