5DN8
1.76 Angstrom Crystal Structure of GTP-binding Protein Der from Coxiella burnetii in Complex with GDP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-02 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.275, 98.443, 107.968 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.050 - 1.760 |
R-factor | 0.17295 |
Rwork | 0.171 |
R-free | 0.20440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2HJC |
RMSD bond length | 0.009 |
RMSD bond angle | 1.473 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.051 | 0.537 |
Number of reflections | 47308 | |
<I/σ(I)> | 33.8 | 3.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | Protein: 11.0 mg/ml, 0.25M Sodium chloride, 0.01M Tris-HCl pH 8.3, 10mM GDP, Screen: JSCG+ (H11), 0.2M Magnesium chloride, 0.1M Bis-Tris pH 5.5, 25% (w/v) PEG 3350. |