5DN5
Structure of a C-terminally truncated glycoside hydrolase domain from Salmonella typhimurium FlgJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.700, 61.120, 65.220 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 29.756 - 2.150 |
| R-factor | 0.1789 |
| Rwork | 0.175 |
| R-free | 0.25170 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.470 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.150 | 0.830 |
| Number of reflections | 21763 | |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35 mg/ml protein in 18-22% polyethylene glycol 3350 and 0.25-0.35 M NaI |
