5DN3
Aurora A in complex with ATP and AA35.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.650, 81.650, 136.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.910 - 2.050 |
| R-factor | 0.1921 |
| Rwork | 0.192 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.460 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.048 | 0.623 |
| Number of reflections | 29802 | |
| <I/σ(I)> | 27.7 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 50 mM HEPES, 200 mM magnesium sulfate, 20% PEG 3350 |
