5DMY
Beta-galactosidase - construct 33-930
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 153.040, 52.650, 153.329 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 54.928 - 1.950 |
| R-factor | 0.2016 |
| Rwork | 0.198 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.930 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.366 | |
| Number of reflections | 170170 | |
| <I/σ(I)> | 5.1 | 2.4 |
| Completeness [%] | 95.2 | 87.1 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% w/v PEG 6000, 0.1M MES, 0.2M NaCl, pH6 |
