5DMV

Polo-box domain of Mouse Polo-like kinase 1 complexed with Emi2 (146-177)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-20
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	35.627, 63.329, 56.831
Unit cell angles	90.00, 97.20, 90.00

Refinement procedure

Resolution	28.414 - 2.499
R-factor	0.2156
Rwork	0.212
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.975
Data reduction software	HKL-2000
Refinement software	PHENIX (1.9_1688)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.414	2.600
High resolution limit [Å]	2.499	2.499
Rmerge		0.438
Number of reflections	8266
<I/σ(I)>	8.5	3.47
Completeness [%]	97.9	89.3
Redundancy	4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	10mM MgCl2, 5mM NiCl2, 15% PEG 3350, 0.1 M HEPES