5DMS
Mouse Polo-box domain and Emi2 (169-177)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.820, 70.450, 59.150 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 35.392 - 1.900 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24130 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX (1.9_1688) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.180 | |
| Number of reflections | 36843 | |
| <I/σ(I)> | 21.7 | 6 |
| Completeness [%] | 98.4 | 91.1 |
| Redundancy | 7.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 15% polyethylene glycol (PEG) 3350 |
