5DMG
X-RAY STRUCTURE OF THE FAB FRAGMENT OF THE ANTI TAU ANTIBODY RB86 IN COMPLEX WITH THE PHOSPHORYLATED TAU PEPTIDE (416-430)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.510, 163.053, 70.926 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 46.610 - 2.500 |
| R-factor | 0.21246 |
| Rwork | 0.210 |
| R-free | 0.26292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house Fab fragment |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.610 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.680 | |
| Number of reflections | 49616 | |
| <I/σ(I)> | 9.73 | 1.17 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.46 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | Lithium sulfate, Sodium acetate, pH 4.5 PEG8000 |
