5DMD
Crystal structure of the flagellar assembly factor FliW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.87260 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.539, 42.516, 71.917 |
Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
Resolution | 46.383 - 1.450 |
R-factor | 0.2062 |
Rwork | 0.205 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aj7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.138 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.380 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.056 | 0.555 |
Number of reflections | 49625 | |
<I/σ(I)> | 9.5 | 1.9 |
Completeness [%] | 99.4 | 96.6 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 40 % (v/v) PEG 400, 5 % (w/v) PEG 3000 |