5DM6
Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2011-03-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.900, 410.760, 696.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.960 - 2.900 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.960 | 58.960 | 3.060 |
| High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
| Rmerge | 0.241 | 0.049 | 7.862 |
| Rmeas | 0.247 | 0.050 | 8.045 |
| Total number of observations | 11664835 | ||
| Number of reflections | 531662 | 17510 | 78466 |
| <I/σ(I)> | 10.38 | 48.65 | 0.71 |
| Completeness [%] | 99.8 | 99.4 | 99.6 |
| Redundancy | 21.9 | 22.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES, sodium hydroxide, magnesium chloride, ammonium chloride, 2-mercaptoethanol, spermidine, 2-ethyl-1, 3-hexanediol, ethanol |
