5DM6
Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2011-03-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.900, 410.760, 696.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.960 - 2.900 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.27030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.514 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.960 | 58.960 | 3.060 |
High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
Rmerge | 0.241 | 0.049 | 7.862 |
Rmeas | 0.247 | 0.050 | 8.045 |
Total number of observations | 11664835 | ||
Number of reflections | 531662 | 17510 | 78466 |
<I/σ(I)> | 10.38 | 48.65 | 0.71 |
Completeness [%] | 99.8 | 99.4 | 99.6 |
Redundancy | 21.9 | 22.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES, sodium hydroxide, magnesium chloride, ammonium chloride, 2-mercaptoethanol, spermidine, 2-ethyl-1, 3-hexanediol, ethanol |