5DLO
S. aureus MazF in complex with substrate analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 64 |
| Unit cell lengths | 63.600, 63.600, 69.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.800 - 1.401 |
| R-factor | 0.1344 |
| Rwork | 0.133 |
| R-free | 0.16340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mzm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 31425 | |
| <I/σ(I)> | 21.64 | 2.03 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 75 mM Tris pH 8.5, 18.75% v/v tert-Butanol, 25% Glycerol |
