5DLJ

Crystal Structure of Cas-DNA-N1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	69.894, 193.652, 193.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.835 - 2.600
R-factor	0.1808
Rwork	0.179
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.050
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.088	0.040	0.807
Total number of observations	499478
Number of reflections	82019
<I/σ(I)>	8.9
Completeness [%]	99.9	99.7	99.8
Redundancy	6.1	6.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	10% PEG400,50mM Na cacodylate,3mM spermine,15mM Magnesium chloride