5DLB
Crystal structure of chaperone EspG3 of ESX-3 type VII secretion system from Mycobacterium marinum M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0702 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.206, 46.019, 58.006 |
Unit cell angles | 90.00, 92.24, 90.00 |
Refinement procedure
Resolution | 57.960 - 1.770 |
R-factor | 0.2108 |
Rwork | 0.209 |
R-free | 0.24460 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 2.130 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.960 | 57.960 | 1.660 |
High resolution limit [Å] | 1.770 | 4.370 | 1.550 |
Rmerge | 0.066 | 0.030 | 1.646 |
Rmeas | 0.063 | 0.043 | 2.221 |
Total number of observations | 137146 | ||
Number of reflections | 25746 | 3043 | 12979 |
<I/σ(I)> | 12.6 | 22.08 | 0.39 |
Completeness [%] | 89.0 | 81 | 85.1 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1uL 21.5mg/mL protein plus 0.5uL reservoir solution drop against 500uL reservoir solution of 1.45M ammonium sulfate, 200mM lithium sulfate, 70mM CAPS pH10.5, microseeded |