5DLA

Structure of Tetragonal Lysozyme solved by UWO Students

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	78.938, 78.938, 36.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.410 - 1.220
R-factor	0.1382
Rwork	0.137
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dl9
RMSD bond length	0.006
RMSD bond angle	1.049
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.410
High resolution limit [Å]	1.220
Rmerge	0.074
Number of reflections	34007
<I/σ(I)>	43.4
Completeness [%]	96.5
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	Protein Solution (60 mg/ml protein in 0.1M Na acetate pH 4.6) mixed in a 1:1 ratio with the Well Solution (10% NaCl buffered in 0.1 M Na acetate pH 4.5). Cryoprotected in 10% NaCl, 0.1 M Na acetate pH 4.5 25% ethylene glycol