5DL2
Crystal Structure of RopB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 94.060, 94.060, 178.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.000 - 3.500 |
R-factor | 0.2796 |
Rwork | 0.276 |
R-free | 0.31340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.865 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 81.000 |
High resolution limit [Å] | 3.500 |
Number of reflections | 13475 |
<I/σ(I)> | 12.5 |
Completeness [%] | 97.4 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | Potassium formate, PEG2000, potassium chloride, Tris-HCl |