5DKY
Crystal structure of glucosidase II alpha subunit (DNJ-bound from)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97946 |
Spacegroup name | H 3 2 |
Unit cell lengths | 189.523, 189.523, 157.784 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.156 |
Rwork | 0.155 |
R-free | 0.18400 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.427 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.086 | 0.419 |
Number of reflections | 141836 | |
<I/σ(I)> | 23.6 | 2.8 |
Completeness [%] | 98.3 | 98 |
Redundancy | 2.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2 M sodium citrate tribasic, 0.1 M Tris-HCl (pH 7.0), 2.5 mM DNJ |