5DKO
The structure of Escherichia coli ZapD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97888 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 108.900, 108.900, 106.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.525 - 2.400 |
R-factor | 0.2511 |
Rwork | 0.247 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oez |
RMSD bond length | 0.003 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.760 | |
Number of reflections | 26464 | |
<I/σ(I)> | 13.7 | 2.3 |
Completeness [%] | 95.2 | 96.1 |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15 mg/ml ZapD with 1.2 M ammonium sulfate, 100 mM HEPES, pH 7.5, 10 % PEG 600 |