5DKF

Reaction of phosphorylated CheY with imidazole 3 of 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-14
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.580, 53.740, 162.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.300 - 1.940
R-factor	0.1579
Rwork	0.157
R-free	0.17750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.672
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	81.300
High resolution limit [Å]	1.940
Number of reflections	37450
<I/σ(I)>	16
Completeness [%]	99.6
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	MnCl2, BeCl2, NaF, imidazole, Tris, ammonium sulfate, glycerol.