5DJY

Fc Heterodimer Design 20.8 Y349S/T366V/K370Y/K409V + E357D/S364Q/Y407A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2013-11-20
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	60.691, 67.279, 78.661
Unit cell angles	90.00, 105.46, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.22335
Rwork	0.221
R-free	0.27665
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.527
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	67.279	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge		0.032	0.632
Rmeas	0.120
Rpim	0.062	0.021	0.453
Total number of observations	122359	3702	17557
Number of reflections	33291
<I/σ(I)>	8.9	22.9	1.9
Completeness [%]	99.6	96	99.9
Redundancy	3.7	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Tris HCl pH 8.5 + 20% PEG 10K