5DJW
Crystal structure of Family 31 alpha-glucosidase (BT_3299) from Bacteroides thetaiotaomicron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-17 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.260, 74.860, 94.430 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Resolution | 47.550 - 2.700 |
R-factor | 0.3329 |
Rwork | 0.330 |
R-free | 0.38970 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.719 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.550 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 34303 | |
<I/σ(I)> | 4.79 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05 M Ammonium Sulfate, 0.05 M Bis-Tris:HCl pH 6.5 30% (v/v), Pentaerythritol Ethoxylate (15/4 EO/OH) |