5DJU
Crystal structure of LOV2 (C450A) domain in complex with Zdk3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.240, 74.500, 81.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.252 - 2.100 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WKQ and a homology model based on 1Q2N |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.119 | 0.374 |
| Number of reflections | 26342 | |
| <I/σ(I)> | 6.7 | 2.1 |
| Completeness [%] | 96.9 | 88.3 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M citric acid pH 4.0, 1.0 M lithium chloride, 18% (w/v) PEG 6000 |
