5DJU

Crystal structure of LOV2 (C450A) domain in complex with Zdk3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00005
Spacegroup name	P 21 21 21
Unit cell lengths	74.240, 74.500, 81.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.252 - 2.100
R-factor	0.2075
Rwork	0.205
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2WKQ and a homology model based on 1Q2N
RMSD bond length	0.008
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.119	0.374
Number of reflections	26342
<I/σ(I)>	6.7	2.1
Completeness [%]	96.9	88.3
Redundancy	3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M citric acid pH 4.0, 1.0 M lithium chloride, 18% (w/v) PEG 6000