5DJS
Thermobaculum terrenum O-GlcNAc transferase mutant - K341M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.188, 216.361, 216.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.800 |
| R-factor | 0.2193 |
| Rwork | 0.218 |
| R-free | 0.24568 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 8.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 82234 | |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 12.5% poly[acrylic acid sodium salt] 2100, 0.5 M ammonium phosphate, 0.1 M strontium chloride |
