5DJO

Crystal structure of the CC1-FHA tandem of Kinesin-3 KIF13A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	99.154, 50.014, 66.941
Unit cell angles	90.00, 91.97, 90.00

Refinement procedure

Resolution	40.488 - 1.740
R-factor	0.1656
Rwork	0.163
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fm8
RMSD bond length	0.008
RMSD bond angle	1.063
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	1.790
High resolution limit [Å]	1.740	1.740
Number of reflections	33409
<I/σ(I)>	12.2	2.6
Completeness [%]	99.0	99.7
Redundancy	5.1	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2 M calcium acetate, 16% (w/v) PEG 3350