5DJN
Crystal structure of the Kinesin-3 KIF13A NC-CC1 mutant - Deletion of P390
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.620, 158.620, 82.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.790 - 2.820 |
| R-factor | 0.1892 |
| Rwork | 0.184 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.123 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 9716 | |
| <I/σ(I)> | 18.7 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 289 | 1.7M ammonium sulphate, 8% (v/v) isopropanol |
