5DJE
Crystal structure of the zuotin homology domain (ZHD) from yeast Zuo1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.480, 70.260, 95.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.675 - 1.850 |
R-factor | 0.1765 |
Rwork | 0.174 |
R-free | 0.21970 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.241 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.100 | 1.490 |
Number of reflections | 31477 | |
<I/σ(I)> | 11.93 | 1.21 |
Completeness [%] | 99.7 | 99 |
Redundancy | 6.9 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | protein at 15 mg/ml was dissolved in 100 mM NaCl, 5 mM HEPES, 5 mM DTT, pH 7.5. One microliter of protein solution was mixed with one microliter of reservoir solution, 30% PEG 3350, 30 mM MgCl2, 100 mM BisTris, pH 6.5, 5 mM DTT and equilibrated against the reservoir at 4C (277K) |