5DJB
Structure of the Haliangium ochraceum BMC-H shell protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 3 |
Unit cell lengths | 69.200, 69.200, 120.418 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.350 - 1.798 |
R-factor | 0.1757 |
Rwork | 0.170 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i6p |
RMSD bond length | 0.003 |
RMSD bond angle | 0.726 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.350 | 1.860 |
High resolution limit [Å] | 1.798 | 1.800 |
Rmerge | 0.150 | 0.828 |
Number of reflections | 118444 | |
<I/σ(I)> | 10.3 | 2.2 |
Completeness [%] | 99.4 | 94.6 |
Redundancy | 5.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1 M ammonium tartrate, pH 6.5, 4% (v/v) formamide |