5DJ6
Fc Heterodimer Design 6.1 F405W/Y407A + T366M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2013-10-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.104, 67.903, 78.765 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.23743 |
| Rwork | 0.235 |
| R-free | 0.28234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.562 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.903 | 67.903 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.077 | 0.464 | |
| Rmeas | 0.156 | ||
| Rpim | 0.081 | 0.048 | 0.322 |
| Total number of observations | 142042 | 4453 | 17977 |
| Number of reflections | 40729 | ||
| <I/σ(I)> | 5.1 | 9.6 | 2.2 |
| Completeness [%] | 97.7 | 92.4 | 94.9 |
| Redundancy | 3.5 | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 20% PEG 3350 + 200mM Magnesium Formate dihydrate |
