5DJ3
Structure of the PLP-Dependent L-Arginine Hydroxylase MppP with D-Arginine Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.718, 108.277, 195.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.530 - 2.227 |
R-factor | 0.1637 |
Rwork | 0.162 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Holo-MppP (5DJ1) |
RMSD bond length | 0.012 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 (705b) |
Data scaling software | HKL-2000 (705b) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.530 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.106 | 0.726 |
Number of reflections | 79816 | |
<I/σ(I)> | 10 | 1.9 |
Completeness [%] | 90.2 | 91.2 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% PEG 550 MMe, 50mM MgCl2, 100mM HEPES pH 8.0 |