5DJ2

Fc Heterodimer Design 7.4 Y407A + T366V/K409V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	193
Detector technology	CCD
Collection date	2013-09-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	60.394, 67.242, 78.766
Unit cell angles	90.00, 104.89, 90.00

Refinement procedure

Resolution	30.000 - 2.560
R-factor	0.19034
Rwork	0.187
R-free	0.24307
RMSD bond length	0.007
RMSD bond angle	1.228
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	67.242	2.700
High resolution limit [Å]	2.560	8.100	2.560
Rmerge		0.041	0.467
Rmeas	0.131
Rpim	0.068	0.028	0.314
Total number of observations	72742	2194	10808
Number of reflections	19661
<I/σ(I)>	8.9	18.9	2.3
Completeness [%]	99.0	95.9	100
Redundancy	3.7	3.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	17% PEG 3350 + 100mM Magnesium Formate dihydrate