5DJ2
Fc Heterodimer Design 7.4 Y407A + T366V/K409V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2013-09-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.394, 67.242, 78.766 |
| Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.560 |
| R-factor | 0.19034 |
| Rwork | 0.187 |
| R-free | 0.24307 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.228 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 67.242 | 2.700 |
| High resolution limit [Å] | 2.560 | 8.100 | 2.560 |
| Rmerge | 0.041 | 0.467 | |
| Rmeas | 0.131 | ||
| Rpim | 0.068 | 0.028 | 0.314 |
| Total number of observations | 72742 | 2194 | 10808 |
| Number of reflections | 19661 | ||
| <I/σ(I)> | 8.9 | 18.9 | 2.3 |
| Completeness [%] | 99.0 | 95.9 | 100 |
| Redundancy | 3.7 | 3.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 17% PEG 3350 + 100mM Magnesium Formate dihydrate |
