5DJ1

Structure of the PLP-Dependent L-Arginine Hydroxylase MppP Holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98178
Spacegroup name	P 21 21 21
Unit cell lengths	85.718, 108.277, 195.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.450 - 2.102
R-factor	0.1449
Rwork	0.144
R-free	0.17110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Streptomyces globisporus MppP
RMSD bond length	0.010
RMSD bond angle	1.059
Data reduction software	HKL-2000 (705)
Data scaling software	HKL-2000 (705)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2148: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.106	0.734
Number of reflections	106188
<I/σ(I)>	30.7	5.2
Completeness [%]	100.0	100
Redundancy	14.4	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	30% PEG 550 MMe, 50mM MgCl2, 100mM HEPES pH 8.0