5DIY
Thermobaculum terrenum O-GlcNAc hydrolase mutant - D120N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-01-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.176, 52.550, 131.511 |
| Unit cell angles | 90.00, 122.35, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.060 |
| R-factor | 0.20238 |
| Rwork | 0.201 |
| R-free | 0.23633 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.179 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.072 | 0.690 |
| Number of reflections | 57769 | |
| <I/σ(I)> | 12.5 | 1.8 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 38% PEG-4000, 400 mM sodium acetate, 0.1 M Tris-HCl |
